Intracellular Levels of IL-10 and STAT3 in Patients with Chronic Lymphocytic Leukemia

Objective: Chronic Lymphocytic Leukemia (CLL) is characterized by the accumulation of CD5+CD19+ B cells in bone marrow and peripheral blood. Recent studies indicated that expression IL-10, AID mir-155 which are regulated STAT3 increased CLL patients. regulator (Breg) secrete IL-10 suppress immune system. While show similar immunophenotypic properties to Breg cells, they also thought be functionally similar. In this study, levels patients were investigated.
 Methods: Peripheral blood samples obtained from (n:24) healthy controls (n:14). mononuclear cultured for 48 hours presence absence CpG with without PMA expression. analyzed anti-CD5, anti-CD19, anti-CD38, anti-STAT3 anti-IL-10 monoclonal antibodies using flow cytometry.
 Results: Compared subjects, IL-10+, IL-10+CD19+, STAT3+CD19+ lymphocyte population Increased was showed CD19+ Our results had no significant difference between whereas found lower patient compared controls.
 Conclusion: These made us clearly different normal lymphocytes might have a role biology CLL. It believed presented data will contribute scrutinize similarity cells.